What Is Prestained Protein Ladder?

Description

Prestained Protein Ladder, Broad Ambit is a admixture of antiseptic proteins covalently accompanying to a dejected dye that resolves to 8 bands amid 7 and 175 kDa if electrophoresed (1). The protein concentrations are anxiously counterbalanced for even intensity. The covalent coupling of the dye to the proteins affects their electrophoretic behavior in SDS-PAGE gels about to austere proteins. The credible atomic weight of the prestained proteins are accustomed in the table below.

The Thermo Scientific PageRuler Plus Prestained Protein Ladder is a admixture of 9 blue-, orange-, and green-stained recombinant proteins (10 to 250 kDa) for use as admeasurement standards in protein electrophoresis (SDS-PAGE) and Western blotting.

This prestained protein MW brand is advised for ecology the advance of SDS-polyacrylamide gel electrophoresis, for assessing alteration ability assimilate PVDF, nylon and nitrocellulose membranes, and for ciphering the almost admeasurement of afar proteins (see References 1-3) that accept been fabricated arresting with gel stains or Western blemish apprehension reagents. A dejected chromophore is apprenticed to all proteins, except proteins of two advertence bands of 70 kDa and 25 kDa that are black with an orange dye and one blooming advertence bandage of 10 kDa. PageRuler Plus Prestained Protein Ladder is accessible to use: no heating, added dilution, or accession of a abbreviation abettor is appropriate afore loading assimilate a gel.

Highlights

Size ambit – nine proteins spanning 10 to 250 kDa

Ready-to-use – supplied in a loading absorber for absolute loading on gels, no charge to boil

Sharp bands – color-coded bands of agnate intesity for simple visualization

Quality activated – anniversary lot evaluated by SDS-PAGE and Western blotting

Bright advertence bands – orange at 70 and 25 kDa, and blooming at 10 kDa

Membrane-compatible – black bands alteration to membranes for Western blotting

Applications

Monitoring protein clearing during SDS-polyacrylamide gel electrophoresis

Monitoring protein alteration assimilate membranes afterwards Western blotting

Sizing of proteins on SDS-polyacrylamide gels and Western blots

Note

For clearing patterns beneath altered gel conditions, see Specifications.

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