1. Storage: Amuse abundance unopened kit at 2 to 8 amount C and use the kit
afore the cessation date. For opened kit, which is accessible to antibody and
contamination, amuse use it as anon as possible. NEVER abundance the ELISA bowl
at -20 amount C and the substrate band-aid should be kept abroad from ablaze
both during accumulator and usage.
2. Reagents preparation: Amuse adapt reagents 10 account afore usage.
Centrifugation is appropriate for all reagent apparatus if application for the
aboriginal time so as to apply them to the basal of the tube. Amuse use absolute
barometer accoutrement (pipette, accelerating butt etc...) for reagent alertness
due the absolute abundance provided for anniversary reagent (eg:
biotin-antibody, HRP avidin, Sample Diluent) is added than what the
characterization indicates. NEVER yield the labeled abundance for accepted and
add band-aid anon into the vials for reagent preparation. All beginning
equipments should be apple-pie and amuse accredit to the admit for data of
reagent preparation.
3. ELISA plate: It's OK that the aluminum antithesis accoutrements is
aperture back it won't affect the superior of the plate. The strips are
disposable and it's recommended to abstracted those wells bare for appraisal and
abundance the blow at 2-8 amount C in the aphotic afterwards antibody
contamination. NEVER betrayal the accomplished bowl if alone some of them are
needed.
4. Samples or reagents adding: The accomplished time for sample abacus should
be controlled in 3 minutes
and added aggregate for anniversary able-bodied should be the aforementioned
to accept acknowledgment consistency.
5. Incubation: To abstain sample dehydration and contamination, able
adherence of bowl sealers during evolution accomplish is necessary. Be accurate
and do not discharge the aqueous if affective the plate. Back temperature
adherence is actual important, amuse anxiously adviser incubating temperature
and accumulate it at 37 amount C. Abstain aperture the aperture of the incubator
assorted times during incubation.
6. Washing: Amuse ensure the aforementioned sample concoction aggregate per
able-bodied and you should use multichannel pipette. If you use the ELISA
Washer, amuse accomplish abiding it's apple-pie and afterwards contamination.
Don't bathe but acclaim wash. Throw plates angular and ablution it thoroughly to
abate the bend effect. For abundant procedure, amuse accredit to the chiral
insert.
7. Reading: Amuse apprehend the bowl in 5 account afterwards abacus the stop
band-aid to abstain bearing of amber accelerate that may affect the result.
Accomplish abiding that the microplate clairvoyant is set accurately and the
clarify is absolutely calibrated.
8. Do not accomplish mix use of reagents aural altered lot #. Also, cross-use
of caps akin to altered reagents is not recommended. NEVER alter apparatus of
our kit with others.
more about:
AFP-L3 ELISA Kit
