Technical Tips of AFP-L3 ELISA Kit

1. Storage: Amuse abundance unopened kit at 2 to 8 amount C and use the kit afore the cessation date. For opened kit, which is accessible to antibody and contamination, amuse use it as anon as possible. NEVER abundance the ELISA bowl at -20 amount C and the substrate band-aid should be kept abroad from ablaze both during accumulator and usage.

2. Reagents preparation: Amuse adapt reagents 10 account afore usage. Centrifugation is appropriate for all reagent apparatus if application for the aboriginal time so as to apply them to the basal of the tube. Amuse use absolute barometer accoutrement (pipette, accelerating butt etc...) for reagent alertness due the absolute abundance provided for anniversary reagent (eg: biotin-antibody, HRP avidin, Sample Diluent) is added than what the characterization indicates. NEVER yield the labeled abundance for accepted and add band-aid anon into the vials for reagent preparation. All beginning equipments should be apple-pie and amuse accredit to the admit for data of reagent preparation.

3. ELISA plate: It's OK that the aluminum antithesis accoutrements is aperture back it won't affect the superior of the plate. The strips are disposable and it's recommended to abstracted those wells bare for appraisal and abundance the blow at 2-8 amount C in the aphotic afterwards antibody contamination. NEVER betrayal the accomplished bowl if alone some of them are needed.

4. Samples or reagents adding: The accomplished time for sample abacus should be controlled in 3 minutes

and added aggregate for anniversary able-bodied should be the aforementioned to accept acknowledgment consistency.

5. Incubation: To abstain sample dehydration and contamination, able adherence of bowl sealers during evolution accomplish is necessary. Be accurate and do not discharge the aqueous if affective the plate. Back temperature adherence is actual important, amuse anxiously adviser incubating temperature and accumulate it at 37 amount C. Abstain aperture the aperture of the incubator assorted times during incubation.

6. Washing: Amuse ensure the aforementioned sample concoction aggregate per able-bodied and you should use multichannel pipette. If you use the ELISA Washer, amuse accomplish abiding it's apple-pie and afterwards contamination. Don't bathe but acclaim wash. Throw plates angular and ablution it thoroughly to abate the bend effect. For abundant procedure, amuse accredit to the chiral insert.

7. Reading: Amuse apprehend the bowl in 5 account afterwards abacus the stop band-aid to abstain bearing of amber accelerate that may affect the result. Accomplish abiding that the microplate clairvoyant is set accurately and the clarify is absolutely calibrated.

8. Do not accomplish mix use of reagents aural altered lot #. Also, cross-use of caps akin to altered reagents is not recommended. NEVER alter apparatus of our kit with others.
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AFP-L3 ELISA Kit