Barbated Skullcup Herb pharmacological effects

1.Bacteriostatic action: these 50% decoction with flat trenching method, for staphylococcus aureus, shigella, salmonella typhi's blessing, pseudomonas aeruginosa, escherichia coli has inhibitory effect.

2. Spasmolysis expectoranting action: this product contains safflower, known as the stronger role in smooth muscle contraction caused by histamine, resistance and good ef-fects.

3. Tumor suppression effect: with methylene blue's test tube method screening test shows that this type of acute myelocytic leukemia cells have a slight inhibition;Using cell respirator screening experiment showed that the type of acute myelocytic leukemia cells inhibition rate greater than 75%.

4. The immune regulating effect: this product contains polysaccharides in vitro can promote mouse spleen lymphocyte cell transformation induced by ConA, the optimum concentration of 400 r/ml.Sc to medicine can obviously improve the peripheral blood lymphocytes in mice after 1 week of esterase the percentage of positive cells, promote the latest hairstyle allergy induced by DNCB.But large dose (200 mg/kg) can inhibit mice thymus index, spleen index had no effect.

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Barbated Skullcup Herb

Bistort Rhizome pharmacological effects

Bistort Rhizome diacolation liquid and gelatin made of hemostatic net 1, for dog and sheep hemostatic experiments (femoral artery to cut off the liver snip, splenectomy and bleeding) all have certain hemostatic effect.In vitro against staphylococcus aureus, pseudomonas aeruginosa, bacillus subtilis, e. coli, etc all have antibacterial activity (flat make hole by). Bistort Rhizome toxicity is very small, with its extract (dew) 100 mice celiac injection of median lethal dose of 0.33 g/rat;Intraperitoneal injection of rabbit hemostatic net (0.2 g/kg), observe the 5 days, anatomy in 30 days, no abnormalities are found.Buried hemostatic net l organization, can absorb.Preliminary evidence that have certain hemostatic anti-inflammatory effects.

Bistort Rhizome diacolation liquid and hemostatic net 1 made of gelatin, etc, used for various dog and sheep hemostatic experiments have certain hemostatic effect.In vitro against staphylococcus aureus, pseudomonas aeruginosa, bacillus subtilis, e. coli, etc all have antibacterial activity.This product was used to extract fluid (100%) injected into mice abdominal cavity injection of the median lethal dose of 0.33 g/kg;Hemostatic net injection of rabbit celiac (0.2 g/kg) observed 5 days, anatomy in 30 days, found no abnormalities, hemostatic net 1 buried organization can absorb.Preliminary evidence that have certain hemostatic anti-inflammatory effects.

Clematis medicinal uses

Clematis root dry root and rhizome of medicine, for clinical medicine of traditional Chinese medicine, with functions of dehumidification, only strengthen the pain, bone infarction, diuresis, all that blood stasis phlegm dampness or as a product can dispel effect and so on, the attending, head wind, cold and dampness, stroke, gout, arthritis pain, obstinate arlhralgia certification, by the wind cold dampness and blood stasis resistance of joint pain, inconvenient flexion, numbness, ribs JuLuan, beriberi, swelling pain, swelling and phlegm retention accumulation, jaundice, bone stuck throat buildup, malaria, remove lump, diphtheria, tetanus, gallstones, urinary stones and outside for mumps and the symptom such as mastitis, the waist and knee, leg pain, effect is significant. Modern pharmacological studies have shown that clematis root has antibacterial, anti-inflammatory, anti-tumor, malaria, fall blood pressure, fall blood sugar, promoting bile secretion, analgesia, spasmolysis, clinical application in rheumatoid arthritis, chronic cholecystitis, strain of lumbar muscles, used in the treatment such as strep throat, heel pain, toothache. This go up character, long take easy injury and bones and muscles JuLuan pain due to blood deficiency are prohibited, as the weak, the weak body empty gas, Yin deficiency heat, no cold do not taken the origin and pregnant women. Fresh whole plant can cure sore throat and acute tonsillitis, root can treat filariasis, external use can be put to a toothache.

Aprotinin storage and solubility

Aprotinin is advisedly acrid in baptize (>10 mg/mL) and in aqueous buffers of low ionic strengths. Dilute solutions are about beneath abiding than concentrated ones. Aprotinin solutions are almost stable, although band-aid adherence is awful abased on pH. Solutions can be stored at 4°C for up to one week. For continued appellation storage; accession of a carrier protein (ex: 0.1% BSA) is recommended, abundance at -20°C. Avoid freeze-thaw cycles.

The Cys14-Cys38 disulfide arch is readily breach by abbreviation agents like b-mercaptoethanol. Due to its bunched tertiary structure, aprotinin is almost abiding adjoin denaturation due to top temperature, acids, alkalies, amoebic solvents or proteolytic abasement (only thermolysin has been begin able of aspersing aprotinin afterwards heating to 60-80°C). The top basicity of aprotinin causes it to attach to frequently acclimated dialysis tubing and even gel filtration matrices, but the use of acetylated abstracts and concentrated alkali solutions minimizes the problem. Sterilization may be accomplished by filtration through a 0.2 mm filter.