2011/08/30

what is The ELISA Kit?

ELISA stands for Enzyme Linked Immuno Sorbent, and to this day, the ELISA kit is the safest and quickest way to diagnose various types of infectious diseases in humans. The ELISA kit, in one of its many forms can also be used to diagnose various diseases in plants and animals as well. The ELISA test can similarly be regarded as the pregnancy test, because it pretty much works the same way. The most common use for the ELISA kit is the detection of antibodies and antigens in samples in the field of immunology. Apart from using the ELISA kit for toxicology tests that quickly detect the presence or absence of certain drugs cause.
Another field in which the ELISA kit has become increasingly important in the food industry. Industry experts use the ELISA kit for the search for various items that do not want to appear on certain products. For example, milk, tree nuts, peanuts, eggs and other ingredients to make similar cause allergic reactions, and a product that does not advertise with one or more of these elements test, tests performed using the ELISA kit. Apart from the fact that in the food industry and law enforcement or competitive ELISA, the kit widely known for his famous used to detect HIV and other viruses. One of the biggest advantages of using the ELISA kit, apart from the exact results is the low price of this product. The financial aspect, together with the short test phase, the ELISA kit to run into one of the most commonly used test methods in the world. Regardless of whether used in diagnostics, plant pathology or product quality control, the ELISA kit in the same way works.
When performing an ELISA test kit antigen is distributed on a surface. In the next step, an antibody that is specific to this test, the antigen is presented in a bind between the two compounds. The final steps are left, the antibody with an enzyme and then adding an insightful solution that helps the enzyme to convert a visible signal. This readable signal will reveal the exact amount of antigen in this specific sample. Since the ELISA test kit for the presence of antibodies and antigen shows, and so it can be used to determine the exact concentration of serum antibodies, it has become one of the most important tools in the correct recognition and diagnosis of HIV virus and also other viruses such as West Nile virus. The high sensitivity of the ELISA test kit has been presented many times that, compared with inspections carried out by using the available microscope.
Using ELISA kits both diagnosis and monitoring of infectious diseases like West Nile virus or HIV. The accuracy of the ELISA kits and the short time of reaction are its strongest selling points. ELISA kits are also very sensitive to specific antibodies and analytical systems to detect very small amounts of chemicals. The high sensitivity of the ELISA kits is due to the strong catalytic ability of enzymes. ELISA methods are also very selective because of the exceptional abilities of discriminatory antibodies. Concentration as low as parts per billion (ppb) can be detected. In addition, an ELISA kit very easy to use and the results are easy to understand.
Commercial uses of the ELISA kits are in high demand. Over-the-counter pregnancy tests use this principle by using monoclonal antibodies against the protein in the urine samples. Diabetes to monitor blood sugar levels can with the help of a mediator molecule interface, biological and electronic components of a sensor, the current results in a reading. Antibiotic residues in milk can be made using an ELISA kit, which inhibit the growth of a test organism can be monitored. ELISA kits are used in the field of food safety by the presence of salmonella. This test is used to predict Salmonella carrier status in cattle. ELISA kits have a huge impact on modern medicine.
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Why is the world ELISA kit is the most widely used one of the test tool


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