Aflatoxin use

(1) the application of food hygiene inspection. China in the mid-1990s, also carried out by ELISA AFB 1

applied research [6-8], and achieved good results, showing that the method is simple, less time-

consuming, specificity, high sensitivity For a large number of samples is particularly suitable for the

screening test.
ELISA using monoclonal antibody method and thin-layer chromatography contrast grain samples tested, TLC

assay showed that the sensitivity of the method under (5ng / g) of the seized samples detected no AFB 1.

These samples by ELISA assay, AFB l detection rate of 96% content of 0.05 ~ 2.20ng / g between. ELISA

method of detection sensitivity much higher than the TLC method.
That AFB l indirect competitive inhibition ELISA (IDC-ELISA) quantitative analysis method is more

conventional TLC method is simple, economic characteristics, it is not only suitable for rapid detection

of large quantities of samples, and has put into commercial production and the “home test” development

potential.
Enzyme-linked immunosorbent assay determination in food AFB l, linear range of 0.25 ~ 5.0ng/ml, the

detection sensitivity of 0.015μg/kg, TLC increased by 300 compared to 400 times greater than the 89.2

recovery, precision greater than 7.67%. The entire measurement process only 4h, greatly reducing the

detection time.
Also by enzyme-linked immunosorbent assay (ELISA) detection of 106 cases of Qidong region of AFT

content of corn samples, and with the conventional thin-layer chromatography were compared to ELISA

method proved superior to thin-layer chromatography. ELISA assay were higher than thin-layer

chromatography, the phenomenon of false negative and did not show specificity by ELISA and high

sensitivity. The method for food hygiene inspection, food for the liver High Risk monitoring and mildew

in the AFT research and other drugs to provide a good technical means, with a good prospect.
(2) in the etiology and prevention of liver cancer in the application. So far, AFT-induced liver cancer

in animal experiments of the study have no doubt. However, studies in humans due to lack of direct

evidence is still not fully sure, but a common human food contamination in the AFT is a human liver

cancer may be factors that view has been widely recognized. Now that the determination of AFT albumin

(AF-Alb) compounds can be used as indicators of intake of AFT’s body; and confirmed by numerous studies

worldwide AF-Alb is a good level of exposure used to estimate the AFT mark.

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