Koichi Ogata, who in 1969 first discovered some of the yeast can use methanol as the sole carbon and energy growth (Ogata, et a1.1969), then, the use of methanol-based yeast single cell protein production potential as animal feed it caused widespread concern. 1987, Cregg, who first reported the use of methanol nutritional yeast expression hepatitis B surface antigen (HbsAg then Philip Petroleum Company and the Salk Institute Biotechnology / Industrial Associates (SIBIA) began Pichia pastoris expression system to develop. SIBIA . AOX researchers isolated the gene promoter and host strains to construct a carrier, and develop the corresponding techniques of genetic manipulation of Pichia pastoris, with Philip Petroleum company production of single-cell protein fermentation process to achieve a foreign protein highly expressed in 1993, Philip Petroleum company will Pichia pastoris expression system of patents the company sold to Research Corporation Technologies, and commissioned Invitrogea companies on product sales.
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